Cq, quantification cycle RFU, relative fluorescence units. Confidently analyze data from a broad range of sample concentrations even when multiplexing five targets. You may start a run on one instrument while another is running a different experiment, or you can start the same experiment on all four instruments at once.
Cfx manager software data analysis series#
A System That Grows with Your Research The flexibility of the series platform allows your setup to grow and evolve as your needs change.Īdding more instruments to increase throughput or expand options is as easy as connecting a USB cable. This substantially lengthens the life of TEs and guarantees outstanding thermal performance. Condensation from constant heating and cooling can degrade thermal electric modules TEscausing them to fail. O-ring hermetic seal extends the life of the heating and cooling elements. Thermal gradient experiment for optimizing annealing temperature. The thermal cycler provides exceptional temperature uniformity and reproducibility within each gradient zone, and the temperatures can easily be programmed and viewed on the touchscreen or onscreen in the software so you can quickly identify the optimal incubation temperature. With the CFX96 Touch systems' thermal gradient feature, you can determine the optimal temperature for primer annealing in a single experiment, minimizing the use of precious samples and reagents and saving valuable research time. Efficient Optimization Determining the optimal temperature for primer annealing is crucial for efficient and specific amplification of product. Note the consistent high average ramp rate throughout heating and cooling. The traces are nearly indistinguishable due to the tight uniformity.
This graph shows the temperature measured by probes in 15 wells across a sample block. Superior uniformity with rapid arrival at target temperature. Now you can tailor your runs around your schedule instead of tailoring your schedule around your runs. Run times can be dramatically shortened - to less than 30 min - while still producing accurate quantitative results. The CFX96 Touch system produces high average ramp rates and tight uniformity during ramping to yield fast time to target temperature and faster protocol run times. Maximum ramp rate is less important because it can fluctuate significantly during the ramp. Rapid Arrival at Target Temperature A key component of overall protocol run time is the time required to reach target temperature, which is determined by the average ramp rate and the time needed for the sample block to reach thermal uniformity. To obtain reliable, consistent results, all sample wells must maintain proper temperature throughout each incubation step. Superior Uniformity Precision of the temperature steps is critical for the rate and efficiency of PCR. Reduced-mass sample block - fast ramping and settling produce the shortest time to target temperature available in a thermal cycler. Thermal gradient feature - identifies optimal annealing temperature in a single run. Protocol autowriter - generates an optimal protocol based on your polymerase, primers, and product length. Bio-Rad engineers have developed a complete thermal cycling system that will speed every step of the PCR process to give you what's really important - a shorter time from setup to results. Many factors contribute to the overall time it takes to get PCR results: the time to design, program, and optimize a protocol, the time to run the protocol, and the time to analyze the results.
If you're like most researchers, you want to get results faster. The optical filter sets are designed to maximize fluorescence detection for specific dyes in specific channels.Īt every position and with every scan, the optics shuttle is reproducibly centered above each well, so the light path is always fixed and optimal, and there is no need to sacrifice data collection in one of the channels to normalize to a passive reference. The optical system automatically collects data from all wells during data acquisition, so you can enter or edit well information on your own schedule. Scanning just above the sample plate, the optics shuttle individually illuminates and detects fluorescence from each well with high sensitivity and no cross talk. Please select your location to view the products, information, and services available, including news, promotions and events.